Hotspot

trnF-GAA-ndhJ

LSC · 47657-48293 · 637 bp

Back to Prunella

Pi 0.00157

MarkerSeek score 59.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 1
pi: 0.00157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	778-779 bp	1.0	80.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	742-743 bp	1.0	79.6
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	779-780 bp	1.0	79.4
trnF-GAA-ndhJ_p4	GTGGGATGCTAGATTGGAGC	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.1	58.0	806-807 bp	1.0	79.1
trnF-GAA-ndhJ_p5	GGAGCAAATGGTCGGGATAG	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.1	58.0	791-792 bp	1.0	79.1