Hotspot

trnS-GGA-rps4

LSC · 44719-44999 · 281 bp

Back to Prunella

Pi 0.00712

MarkerSeek score 56.60

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 2
pi: 0.00712
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TGACTCTTCACCCAGTCCAA	50.0	50.0	58.1	58.2	437 bp	1.0	78.0
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	TTGACTCTTCACCCAGTCCA	50.0	50.0	58.1	58.2	438 bp	1.0	78.0
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	TGACTCTTCACCCAGTCCAA	50.0	50.0	58.4	58.2	436 bp	1.0	75.7
trnS-GGA-rps4_p4	GGCTTTTGTTTACCGAGGGT	TTGACTCTTCACCCAGTCCA	50.0	50.0	58.4	58.2	437 bp	1.0	75.7
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	TTTGACTCTTCACCCAGTCC	50.0	50.0	58.1	57.1	439 bp	1.0	72.2