Hotspot

trnG-GCC-trnfM-CAU

LSC · 35669-35832 · 164 bp

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Pi 0.0124
MarkerSeek score 61.40
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
164
variable_sites
2
pi
0.0124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 227-230 bp 1.0 81.0
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 228-231 bp 1.0 81.0
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 263-266 bp 1.0 80.8
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 264-267 bp 1.0 80.8
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC TGACTTCTTCTCTTCAGCGC 50.0 50.0 58.1 57.9 284-287 bp 1.0 80.4