Hotspot

rpoB-trnC-GCA

LSC · 26517-27684 · 1168 bp

Back to Prunella

Pi 0.00171
MarkerSeek score 59.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1168
variable_sites
2
pi
0.00171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AGTTCTTCTGCCAAACCCTG TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1355 bp 1.0 79.8
rpoB-trnC-GCA_p2 GTTCTTCTGCCAAACCCTGA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1354 bp 1.0 79.8
rpoB-trnC-GCA_p3 AGTTCTTCTGCCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1334 bp 1.0 79.4
rpoB-trnC-GCA_p4 GTTCTTCTGCCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1333 bp 1.0 79.3
rpoB-trnC-GCA_p5 GCCAAACCCTGATCGATGAA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.2 58.0 1346 bp 1.0 77.9