Hotspot

psbA

LSC · 96-1157 · 1062 bp

Back to Protea

Pi 0.00188
MarkerSeek score 48.10
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
2
pi
0.00188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TTGCTCCTTCAATAACTCGT AATTCGTGTGCTTGGGAGTC 40.0 50.0 54.7 58.5 1151 bp 1.0 49.9
psbA_p2 GGTATTGCTCCTTCAATAACTCG AATTCGTGTGCTTGGGAGTC 43.5 50.0 57.3 58.5 1155 bp 1.0 47.1
psbA_p3 TTGCTCCTTCAATAACTCGT TAATTCGTGTGCTTGGGAGT 40.0 45.0 54.7 57.1 1152 bp 1.0 46.9
psbA_p4 ATTGCTCCTTCAATAACTCGT AATTCGTGTGCTTGGGAGTC 38.1 50.0 55.3 58.5 1152 bp 1.0 46.8
psbA_p5 TTGCTCCTTCAATAACTCGT GCTTGGGAGTCCCTGATGAT 40.0 55.0 54.7 59.2 1142 bp 1.0 44.5