Hotspot

ndhD

SSC · 124689-126188 · 1500 bp

Back to Protea

Pi 0.00267
MarkerSeek score 47.70
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1500
variable_sites
4
pi
0.00267
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TCTGGTCAAAGCATATCTTGT GTCTTTCTTATTCATGAAAACTATCT 38.1 26.9 54.9 53.9 1582 bp 1.0 40.2
ndhD_p2 TCTGGTCAAAGCATATCTTGTT GTCTTTCTTATTCATGAAAACTATCT 36.4 26.9 55.7 53.9 1582 bp 1.0 40.2
ndhD_p3 TTCTGGTCAAAGCATATCTTGT GTCTTTCTTATTCATGAAAACTATCT 36.4 26.9 55.7 53.9 1583 bp 1.0 40.2
ndhD_p4 TCTGGTCAAAGCATATCTTGT GTCTTTCTTATTCATGAAAACTATCTT 38.1 25.9 54.9 54.6 1582 bp 1.0 40.2
ndhD_p5 TCTGGTCAAAGCATATCTTGT GTCTTTCTTATTCATGAAAACTATC 38.1 28.0 54.9 52.6 1582 bp 1.0 40.2