Hotspot

trnR-ACG-trnN-GUU

IRb · 111628-112231 · 604 bp

Back to Protea

Pi 0.00662
MarkerSeek score 62.60
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
604
variable_sites
4
pi
0.00662
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 746-752 bp 1.0 78.7
trnR-ACG-trnN-GUU_p2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.7 58.0 783-789 bp 1.0 78.1
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 697-703 bp 1.0 77.5
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 55.0 50.0 57.8 57.8 786-792 bp 1.0 77.1
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG CTGGTCGTAGGTTCGAATCC 55.0 55.0 57.8 57.8 698-704 bp 1.0 77.1