Hotspot

rpl33-rps18

LSC · 70313-70957 · 645 bp

Back to Protea

Pi 0.0157
MarkerSeek score 58.70
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
645
variable_sites
10
pi
0.0157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 ACATACGATTCATGGGGAGA AAGAAAGGGTCGCTTGGATT 45.0 45.0 55.7 57.4 703-708 bp 1.0 59.0
rpl33-rps18_p2 ACATACGATTCATGGGGAGA AGAAAGGGTCGCTTGGATTT 45.0 45.0 55.7 57.4 702-707 bp 1.0 59.0
rpl33-rps18_p3 ACATACGATTCATGGGGAGA ATTCGATCCCCCGATCCAAT 45.0 50.0 55.7 58.6 759-764 bp 1.0 58.5
rpl33-rps18_p4 ACATACGATTCATGGGGAGA ATCAATTCGATCCCCCGATC 45.0 50.0 55.7 57.2 763-768 bp 1.0 57.4
rpl33-rps18_p5 AACATACGATTCATGGGGAGA AGAAAGGGTCGCTTGGATTT 42.9 45.0 56.5 57.4 703-708 bp 1.0 57.4