Hotspot

petA-psbJ

LSC · 65565-66566 · 1002 bp

Back to Protea

Pi 0.022
MarkerSeek score 71.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1002
variable_sites
22
pi
0.022
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.8 57.6 1129-1130 bp 1.0 77.6
petA-psbJ_p2 CCATTACGTGTTCAAGGCCT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1144-1145 bp 1.0 77.4
petA-psbJ_p3 ACCCATTACGTGTTCAAGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.5 57.6 1146-1147 bp 1.0 75.1
petA-psbJ_p4 ACGTGTTCAAGGCCTTTTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 58.5 57.6 1139-1140 bp 1.0 74.6
petA-psbJ_p5 CGTGTTCAAGGCCTTTTGTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.4 57.6 1138-1139 bp 1.0 74.2