Hotspot

psbC-trnS-UGA

LSC · 38599-38812 · 214 bp

Back to Protea

Pi 0.0236
MarkerSeek score 66.90
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
214
variable_sites
5
pi
0.0236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 321 bp 1.0 80.0
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 344 bp 1.0 79.8
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC TCTTTCGGAGAGATGGCTGA 55.0 50.0 58.2 58.1 343 bp 1.0 79.2
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 267 bp 1.0 79.1
psbC-trnS-UGA_p5 CCTGTTCTTTCCATGACCCC CTTTCGGAGAGATGGCTGAG 55.0 55.0 58.2 57.8 342 bp 1.0 78.6