Hotspot

trnD-GUC-psbM

LSC · 31236-32321 · 1086 bp

Back to Protea

Pi 0.00489
MarkerSeek score 66.10
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1086
variable_sites
5
pi
0.00489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 AGTTCAATCGGTTAGAGCACC AGAACGAAGAGTGCAGTAGC 47.6 50.0 58.0 57.6 1136-1199 bp 1.0 73.3
trnD-GUC-psbM_p2 GTTCAATCGGTTAGAGCACC AGAACGAAGAGTGCAGTAGC 50.0 50.0 56.5 57.6 1135-1198 bp 1.0 69.8
trnD-GUC-psbM_p3 TATGTATGCTGTACACCCCC AGAACGAAGAGTGCAGTAGC 50.0 50.0 56.4 57.6 1173-1236 bp 1.0 68.6
trnD-GUC-psbM_p4 AGTTCAATCGGTTAGAGCACC AGGCAGTAGGAACTAGAACGA 47.6 47.6 58.0 57.9 1150-1213 bp 1.0 67.5
trnD-GUC-psbM_p5 AGTTCAATCGGTTAGAGCACC GGAACTAGAACGAAGAGTGCA 47.6 47.6 58.0 57.7 1142-1205 bp 1.0 65.7