Hotspot

trnC-GCA-trnE-UUC

LSC · 29201-30491 · 1291 bp

Back to Protea

Pi 0.00852
MarkerSeek score 63.60
Primer available yes
Label trnC-GCA-trnE-UUC

Feature Metadata

label_name
trnC-GCA-trnE-UUC
feature_type
igs
region
LSC
length_bp
1291
variable_sites
11
pi
0.00852
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnE-UUC_p1 TTTCCCCAGTTCAAATCCGG TTTCGTAGTACCCTACCCCC 50.0 55.0 58.1 57.8 1416-1457 bp 1.0 78.7
trnC-GCA-trnE-UUC_p2 TTTCCCCAGTTCAAATCCGG GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.1 58.3 1378-1419 bp 1.0 77.9
trnC-GCA-trnE-UUC_p3 ACTGCAAATCCCTTTTCCCC TTTCGTAGTACCCTACCCCC 50.0 55.0 58.4 57.8 1429-1470 bp 1.0 76.5
trnC-GCA-trnE-UUC_p4 GCAAATCCCTTTTCCCCAGT TTTCGTAGTACCCTACCCCC 50.0 55.0 58.4 57.8 1426-1467 bp 1.0 76.5
trnC-GCA-trnE-UUC_p5 TGCAAATCCCTTTTCCCCAG TTTCGTAGTACCCTACCCCC 50.0 55.0 58.4 57.8 1427-1468 bp 1.0 76.5