Hotspot

trnS-GCU-trnG-UCC

LSC · 8705-9884 · 1180 bp

Back to Protea

Pi 0.00512
MarkerSeek score 62.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1180
variable_sites
6
pi
0.00512
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGGAAAGAGAGGGATTCGA TGATCAGAATCAAACCGAACCT 50.0 40.9 58.2 57.7 1342-1344 bp 1.0 61.3
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA TGATCAGAATCAAACCGAACC 50.0 42.9 58.2 56.3 1342-1344 bp 1.0 57.7
trnS-GCU-trnG-UCC_p3 CTTTCGTCCACTCAGCCATC TGATCAGAATCAAACCGAACCT 55.0 40.9 58.6 57.7 1278-1280 bp 1.0 57.5
trnS-GCU-trnG-UCC_p4 AACGGAAAGAGAGGGATTCG TGATCAGAATCAAACCGAACCT 50.0 40.9 57.3 57.7 1343-1345 bp 1.0 57.1
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA TTGATCAGAATCAAACCGAACC 50.0 40.9 58.2 57.0 1343-1345 bp 1.0 55.4