Hotspot

psbK-psbI

LSC · 8017-8421 · 405 bp

Back to Protea

Pi 0.00988
MarkerSeek score 52.00
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
405
variable_sites
4
pi
0.00988
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TTCTTTTATTCCCCGCGTCC 50.0 50.0 57.9 58.3 551 bp 1.0 78.1
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT TTCTTTTATTCCCCGCGTCC 45.0 50.0 58.3 58.3 548 bp 1.0 76.0
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT TTCTTTTATTCCCCGCGTCC 45.0 50.0 58.3 58.3 549 bp 1.0 76.0
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT TTCTTTTATTCCCCGCGTCC 45.0 50.0 58.3 58.3 550 bp 1.0 76.0
psbK-psbI_p5 TGTTTGGCAAGCTGCTGTAA TTCTTTTATTCCCCGCGTCC 45.0 50.0 58.6 58.3 552 bp 1.0 73.7