Hotspot

ycf1

SSC · 123180-128747 · 5568 bp

Back to Prostanthera

Pi 0.0252

MarkerSeek score 55.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 237
pi: 0.0252
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCCAACTCCGAGATGCAAT	ACGAGCTCAAAGGCGTAAAA	50.0	45.0	58.0	58.1	990-1005 bp	1.0	88.2
ycf1_p2	GTCCAACTCCGAGATGCAAT	CCGTTCAAGAAAAGGCAAGC	50.0	50.0	58.0	58.2	1128-1143 bp	1.0	86.9
ycf1_p3	TCAGGGGGTTGTGATAGGAG	ACGAGCTCAAAGGCGTAAAA	55.0	45.0	58.1	58.1	459-462 bp	1.0	86.2
ycf1_p4	GTGCCAAGGTTTCAGACAGA	ACGAGCTCAAAGGCGTAAAA	50.0	45.0	58.0	58.1	613-616 bp	1.0	85.8
ycf1_p5	GTCCAACTCCGAGATGCAAT	CGTTCAAGAAAAGGCAAGCC	50.0	50.0	58.0	58.2	1142 bp	0.25	47.9