Hotspot

accD

LSC · 58346-59857 · 1512 bp

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Pi 0.0233

MarkerSeek score 62.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 59
pi: 0.0233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGGGGGCAAGAAAACTCT	AAATAATGGAACTTGGCGCG	50.0	45.0	55.5	57.4	1531-1552 bp	1.0	44.8
accD_p2	AGGGGGCAAGAAAACTCT	AAAATAATGGAACTTGGCGCG	50.0	42.9	55.5	58.1	1532-1553 bp	1.0	44.8
accD_p3	AGGGGGCAAGAAAACTCT	AATAATGGAACTTGGCGCGC	50.0	50.0	55.5	59.9	1530-1551 bp	1.0	44.7
accD_p4	AGGGGGCAAGAAAACTCT	ATAATGGAACTTGGCGCGC	50.0	52.6	55.5	59.3	1529-1550 bp	1.0	44.7
accD_p5	AGGGGGCAAGAAAACTCT	TAAAATAATGGAACTTGGCGCG	50.0	40.9	55.5	58.0	1533-1554 bp	0.75	33.0