Hotspot

trnI-CAU-rpl23

LSC · 150795-150984 · 190 bp

Back to Prostanthera

Pi 0.154

MarkerSeek score 65.00

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 47
pi: 0.154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	GAATGGTTAAAGCGCCCAAC	TTCTACCCTTTCCCGGAAGT	50.0	50.0	58.0	58.0	417-430 bp	1.0	91.7
trnI-CAU-rpl23_p2	GTTGAACCCTGTGAATTGCG	TTCTACCCTTTCCCGGAAGT	50.0	50.0	57.9	58.0	731-752 bp	1.0	91.3
trnI-CAU-rpl23_p3	TAGGAAGAAGCCCCCTCAAA	TTCTACCCTTTCCCGGAAGT	50.0	50.0	57.9	58.0	853-874 bp	1.0	91.3
trnI-CAU-rpl23_p4	GAATGGTTAAAGCGCCCAAC	ACTGTCTACGGTTCCATTGC	50.0	50.0	58.0	57.9	596-609 bp	1.0	91.1
trnI-CAU-rpl23_p5	GAATGGTTAAAGCGCCCAAC	GAAGAGTTCGACCCAATGCT	50.0	50.0	58.0	57.9	362-375 bp	1.0	91.1