Hotspot

ycf2-trnI-CAU

LSC · 150633-150720 · 88 bp

Back to Prostanthera

Pi 0.11

MarkerSeek score 63.40

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 17
pi: 0.11
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GTTGAACCCTGTGAATTGCG	TTCTACCCTTTCCCGGAAGT	50.0	50.0	57.9	58.0	731-752 bp	1.0	91.3
ycf2-trnI-CAU_p2	TAGGAAGAAGCCCCCTCAAA	TTCTACCCTTTCCCGGAAGT	50.0	50.0	57.9	58.0	853-874 bp	1.0	91.3
ycf2-trnI-CAU_p3	GTTGAACCCTGTGAATTGCG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.9	58.0	329-342 bp	1.0	86.2
ycf2-trnI-CAU_p4	TAGGAAGAAGCCCCCTCAAA	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.9	58.0	451-464 bp	1.0	84.8
ycf2-trnI-CAU_p5	GGTATAGGAAGAAGCCCCCT	GTTGGGCGCTTTAACCATTC	55.0	50.0	57.9	58.0	455-468 bp	1.0	84.2