Hotspot

ndhF-rpl32

SSC · 112491-113002 · 512 bp

Back to Prostanthera

Pi 0.0371
MarkerSeek score 68.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
512
variable_sites
30
pi
0.0371
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAAAGGCAAAATCCACGCAT TGATGCAGAAGTACGCTTTT 45.0 40.0 57.6 55.7 584-589 bp 1.0 63.0
ndhF-rpl32_p2 GAAAGGCAAAATCCACGCAT TTGATGCAGAAGTACGCTTT 45.0 40.0 57.6 55.7 585-590 bp 1.0 63.0
ndhF-rpl32_p3 GAAAGGCAAAATCCACGCAT TTTGATGCAGAAGTACGCTT 45.0 40.0 57.6 55.7 586-591 bp 1.0 63.0
ndhF-rpl32_p4 GAAAGGCAAAATCCACGCAT TTTTGATGCAGAAGTACGCT 45.0 40.0 57.6 55.7 587-592 bp 1.0 63.0
ndhF-rpl32_p5 AACGAAAGGCAAAATCCACG TGATGCAGAAGTACGCTTTT 45.0 40.0 57.6 55.7 587-592 bp 1.0 62.6