Hotspot

trnI-CAU-ycf2

LSC · 87094-87181 · 88 bp

Back to Prostanthera

Pi 0.11

MarkerSeek score 63.40

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 17
pi: 0.11
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	TTCTACCCTTTCCCGGAAGT	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	746-753 bp	1.0	89.6
trnI-CAU-ycf2_p2	TTCTACCCTTTCCCGGAAGT	TAGGAAGAAGCCCCCTCAAA	50.0	50.0	58.0	57.9	868-875 bp	1.0	89.2
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	329-342 bp	1.0	86.2
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	TAGGAAGAAGCCCCCTCAAA	50.0	50.0	58.0	57.9	451-464 bp	1.0	84.8
trnI-CAU-ycf2_p5	GTTGGGCGCTTTAACCATTC	GGTATAGGAAGAAGCCCCCT	50.0	55.0	58.0	57.9	455-468 bp	1.0	84.2