Hotspot

rpl23-trnI-CAU

LSC · 86826-87019 · 194 bp

Back to Prostanthera

Pi 0.22
MarkerSeek score 85.80
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
LSC
length_bp
194
variable_sites
70
pi
0.22
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 TCGACCCAATGCTTTAGTTCT GAATGGTTAAAGCGCCCAAC 42.9 50.0 57.3 58.0 363-377 bp 1.0 78.4
rpl23-trnI-CAU_p2 CGACCCAATGCTTTAGTTCTG GAATGGTTAAAGCGCCCAAC 47.6 50.0 57.3 58.0 362-376 bp 1.0 77.9
rpl23-trnI-CAU_p3 TCGACCCAATGCTTTAGTTCT CGCTAAGCATCCCTAAGCAT 42.9 50.0 57.3 57.8 391-405 bp 1.0 77.0
rpl23-trnI-CAU_p4 CGACCCAATGCTTTAGTTCTG CGCTAAGCATCCCTAAGCAT 47.6 50.0 57.3 57.8 390-404 bp 1.0 76.6
rpl23-trnI-CAU_p5 TTCGACCCAATGCTTTAGTTCT GAATGGTTAAAGCGCCCAAC 40.9 50.0 58.0 58.0 364-378 bp 1.0 75.8