Hotspot

petB-petD

LSC · 77249-77434 · 186 bp

Back to Prostanthera

Pi 0.0573

MarkerSeek score 66.60

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 21
pi: 0.0573
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TGCACTTTCCAATGATACGT	GTTCAAGTCACACACTCCCA	40.0	50.0	55.4	57.7	254 bp	1.0	63.0
petB-petD_p2	TGCACTTTCCAATGATACGT	AGTTCAAGTCACACACTCCC	40.0	50.0	55.4	57.4	255 bp	1.0	60.6
petB-petD_p3	ATGCACTTTCCAATGATACGT	GTTCAAGTCACACACTCCCA	38.1	50.0	55.9	57.7	255 bp	1.0	59.5
petB-petD_p4	AATGCACTTTCCAATGATACGT	GTTCAAGTCACACACTCCCA	36.4	50.0	56.7	57.7	256 bp	1.0	57.4
petB-petD_p5	ATGCACTTTCCAATGATACGT	AGTTCAAGTCACACACTCCC	38.1	50.0	55.9	57.4	256 bp	1.0	57.1