Hotspot

rbcL-accD

LSC · 57663-58345 · 683 bp

Back to Prostanthera

Pi 0.0302

MarkerSeek score 66.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 34
pi: 0.0302
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCATGGTATGCACTTCCGTG	TCACTATCACTGCTGTTCGC	50.0	50.0	58.0	58.0	1791-1796 bp	0.5	61.4
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	TCACTATCACTGCTGTTCGC	50.0	50.0	58.0	58.0	1991-1996 bp	0.5	61.3
rbcL-accD_p3	TCTTCACATTCACCGTGCAA	TCACTATCACTGCTGTTCGC	45.0	50.0	58.0	58.0	1839-1844 bp	0.5	61.2
rbcL-accD_p4	TTCTTCACATTCACCGTGCA	TCACTATCACTGCTGTTCGC	45.0	50.0	58.0	58.0	1840-1845 bp	0.5	61.2
rbcL-accD_p5	AATGCTACTGCGGGTACATG	TCACTATCACTGCTGTTCGC	50.0	50.0	58.0	58.0	1988-1993 bp	0.5	61.0