Hotspot

ndhC-trnV-UAC

LSC · 51347-52397 · 1051 bp

Back to Prostanthera

Pi 0.0213
MarkerSeek score 66.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1051
variable_sites
33
pi
0.0213
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.3 57.8 1154-1167 bp 1.0 46.3
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.3 57.5 1153-1166 bp 1.0 46.3
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTTG AGAAGGTCTACGGTTCGAGT 34.8 50.0 54.9 57.8 1154-1167 bp 1.0 46.3
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTTGA GTTCGAGTCCGTATAGCCCT 33.3 55.0 56.3 58.7 1142-1155 bp 1.0 46.3
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGAT AGAAGGTCTACGGTTCGAGT 32.0 50.0 56.8 57.8 1154-1167 bp 1.0 46.3