Hotspot

ycf3-trnS-GGA

LSC · 45071-45905 · 835 bp

Back to Prostanthera

Pi 0.0251

MarkerSeek score 66.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 33
pi: 0.0251
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	AATGCCTCCCTTTCTCCTGA	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.3	58.1	1009-1014 bp	1.0	79.2
ycf3-trnS-GGA_p2	ATGCCTCCCTTTCTCCTGAA	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.3	58.1	1008-1013 bp	1.0	79.2
ycf3-trnS-GGA_p3	AATGCCTCCCTTTCTCCTGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	1030-1035 bp	1.0	78.7
ycf3-trnS-GGA_p4	ATGCCTCCCTTTCTCCTGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	1029-1034 bp	1.0	78.7
ycf3-trnS-GGA_p5	ATGCCTCCCTTTCTCCTGAA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	979-984 bp	1.0	78.5