Hotspot

rps12-clpP

LSC · 77719-77920 · 202 bp

Back to Prosopis

Pi 0.0697

MarkerSeek score 78.70

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 14
pi: 0.0697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTAGTACATGTTCCTCGGCG	TTATGTCAGCAGCAGAAGCC	55.0	50.0	57.9	58.3	367-368 bp	1.0	80.2
rps12-clpP_p2	TGACATTTCGGATTGGCTGT	TTATGTCAGCAGCAGAAGCC	45.0	50.0	57.8	58.3	315-316 bp	1.0	80.2
rps12-clpP_p3	TTTCGGATTGGCTGTCTTGT	TTATGTCAGCAGCAGAAGCC	45.0	50.0	57.7	58.3	310-311 bp	1.0	79.6
rps12-clpP_p4	CTAGTACATGTTCCTCGGCG	TCCGAAGACATGGAAAGGGA	55.0	50.0	57.9	58.3	392-393 bp	1.0	79.3
rps12-clpP_p5	CTAGTACATGTTCCTCGGCG	TACAAAGAACGGGCAAACCT	55.0	45.0	57.9	57.6	424-425 bp	1.0	79.0