Hotspot

rpl33-rps18

LSC · 75522-75867 · 346 bp

Back to Prosopis

Pi 0.0737
MarkerSeek score 76.50
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
346
variable_sites
25
pi
0.0737
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCCTGTTGTTGCAAA TCAATTCGATCCCCCGATTG 45.0 50.0 57.9 57.7 482-506 bp 1.0 82.1
rpl33-rps18_p2 TTCTGTCCCTGTTGTTGCAA TCAATTCGATCCCCCGATTG 45.0 50.0 57.9 57.7 483-507 bp 1.0 82.0
rpl33-rps18_p3 ATTCTGTCCCTGTTGTTGCA TCAATTCGATCCCCCGATTG 45.0 50.0 57.6 57.7 484-508 bp 1.0 80.2
rpl33-rps18_p4 TCTGTCCCTGTTGTTGCAAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 456-480 bp 1.0 79.7
rpl33-rps18_p5 TTCTGTCCCTGTTGTTGCAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 457-481 bp 1.0 79.7