Hotspot

psbE-petL

LSC · 72004-73334 · 1331 bp

Back to Prosopis

Pi 0.055

MarkerSeek score 89.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1331
variable_sites: 72
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.0	58.2	1534-1540 bp	1.0	83.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.9	58.2	1523-1529 bp	1.0	83.1
psbE-petL_p3	TGACAAATAACCAACCCGCA	TCGTATCTTGCTCAGACCGA	45.0	50.0	58.0	58.3	1533-1539 bp	1.0	82.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1446-1452 bp	1.0	82.6
psbE-petL_p5	CAACCCGCAATGAATAGGGA	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.9	58.3	1522-1528 bp	1.0	82.5