Hotspot

petA-psbJ

LSC · 69908-71235 · 1328 bp

Back to Prosopis

Pi 0.0451

MarkerSeek score 87.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1328
variable_sites: 58
pi: 0.0451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCTTGTTCTTCGTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1452-1455 bp	1.0	81.5
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1462-1465 bp	1.0	80.6
petA-psbJ_p3	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1446-1449 bp	1.0	80.5
petA-psbJ_p4	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1447-1450 bp	1.0	80.5
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1467-1470 bp	1.0	80.0