Hotspot

trnF-GAA-ndhJ

LSC · 54741-55440 · 700 bp

Back to Prosopis

Pi 0.0441

MarkerSeek score 82.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 30
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	802-805 bp	1.0	84.5
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	841-1137 bp	1.0	83.9
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	803-806 bp	1.0	83.9
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	842-1138 bp	1.0	83.3
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATGGCCCTTGCGTAAAGATT	55.0	45.0	58.0	57.9	831-1127 bp	1.0	82.9