Hotspot

trnC-GCA-petN

LSC · 31721-33016 · 1296 bp

Back to Prosopis

Pi 0.0803

MarkerSeek score 81.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 98
pi: 0.0803
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1398-1429 bp	1.0	86.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	GCGGTTCCTCATTGTCCATA	50.0	50.0	58.1	57.7	1761-1780 bp	1.0	85.1
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	GCGGTTCCTCATTGTCCATA	50.0	50.0	57.9	57.7	1813-1832 bp	1.0	84.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCCAGGGGTTTTCTATGGGA	50.0	50.0	58.1	57.6	2072 bp	0.5	56.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1419 bp	0.5	56.3