Hotspot

psbI-trnS-GCU

LSC · 9645-9906 · 262 bp

Back to Prosopis

Pi 0.11

MarkerSeek score 76.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 28
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTTTTGTTTGGCAAGCTGCT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.1	58.0	656-661 bp	1.0	84.3
psbI-trnS-GCU_p2	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	651-656 bp	1.0	84.3
psbI-trnS-GCU_p3	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	652-657 bp	1.0	84.3
psbI-trnS-GCU_p4	CTTTTGTTTGGCAAGCTGCT	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.1	58.0	657-662 bp	1.0	84.2
psbI-trnS-GCU_p5	CTTTTGTTTGGCAAGCTGCT	GCCCTTTTCAATTGGGGAGA	45.0	50.0	58.1	58.1	686-691 bp	1.0	83.6