Hotspot

trnQ-UUG-psbK

LSC · 8440-9172 · 733 bp

Back to Prosopis

Pi 0.0792

MarkerSeek score 80.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 50
pi: 0.0792
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	ACAGACCAAACTAAAGATATTAAGCA	50.0	30.8	58.7	56.9	716-798 bp	1.0	49.3
trnQ-UUG-psbK_p2	ACCAAAACCCGTTGCCTTAC	ACAGACCAAACTAAAGATATTAAGCA	50.0	30.8	59.0	56.9	715-797 bp	1.0	49.3
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	ACAAATACAGACCAAACTAAAGA	50.0	30.4	58.7	53.9	722-804 bp	1.0	49.2
trnQ-UUG-psbK_p4	GAATAGCGGGACCAAAACCC	ACAGACCAAACTAAAGATATTAAGCA	55.0	30.8	58.9	56.9	725-807 bp	1.0	49.2
trnQ-UUG-psbK_p5	GAATAGCGGGACCAAAACCC	ACAAATACAGACCAAACTAAAGA	55.0	30.4	58.9	53.9	731-813 bp	1.0	49.2