Hotspot

ycf1

SSC · 124154-129637 · 5484 bp

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Pi 0.0191

MarkerSeek score 51.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 157
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGGAATGTGCATTTGCTTC	TCCCGCGGACAAATTTGTAA	50.0	45.0	58.0	58.1	707 bp	0.666667	66.8
ycf1_p2	AAAAGTGGGGGAATGTGCAT	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	714 bp	0.666667	66.7
ycf1_p3	AAAGTGGGGGAATGTGCATT	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	713 bp	0.666667	66.7
ycf1_p4	AAGTGGGGGAATGTGCATTT	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	712 bp	0.666667	66.7
ycf1_p5	TGCCAAGGTTTCAGACGAAA	TCCCGCGGACAAATTTGTAA	45.0	45.0	58.0	58.1	1208 bp	0.666667	66.5