Hotspot

rpl22

LSC · 83943-84350 · 408 bp

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Pi 0.0229

MarkerSeek score 54.60

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 408
variable_sites: 14
pi: 0.0229
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCCCATAATCCCCCACAAGT	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1217-1240 bp	1.0	82.5
rpl22_p2	TCCCATAATCCCCCACAAGT	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1215-1238 bp	1.0	82.5
rpl22_p3	GAATGATGCCTTTGGGTTGC	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1270-1293 bp	1.0	82.4
rpl22_p4	GAATGATGCCTTTGGGTTGC	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1268-1291 bp	1.0	82.3
rpl22_p5	AGTTTCCGGTTCACAGCATT	GTTGGACAAGTGGGGAATGT	45.0	50.0	58.0	58.0	1534-1557 bp	1.0	82.3