Hotspot

psbA

LSC · 286-1347 · 1062 bp

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Pi 0.00188

MarkerSeek score 36.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTCCTTTATTTTCAAGAACTCA	ATTCATGTGCTTGGGAGTCC	33.3	50.0	56.8	57.9	1162 bp	1.0	40.2
psbA_p2	TTGCTCCTTTATTTTCAAGAACTCA	ATTCATGTGCTTGGGAGTCC	32.0	50.0	57.4	57.9	1163 bp	1.0	40.2
psbA_p3	TGCTCCTTTATTTTCAAGAACTCAT	ATTCATGTGCTTGGGAGTCC	32.0	50.0	57.2	57.9	1162 bp	1.0	40.2
psbA_p4	GCTCCTTTATTTTCAAGAACTCA	ATTCATGTGCTTGGGAGTCC	34.8	50.0	55.2	57.9	1161 bp	1.0	40.2
psbA_p5	TGCTCCTTTATTTTCAAGAACTC	ATTCATGTGCTTGGGAGTCC	34.8	50.0	55.2	57.9	1162 bp	1.0	40.2