Hotspot

rpl32-trnL-UAG

SSC · 113650-114562 · 913 bp

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Pi 0.0268

MarkerSeek score 61.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 913
variable_sites: 35
pi: 0.0268
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAAGGATATTGGGCAGCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1033-1037 bp	1.0	83.3
rpl32-trnL-UAG_p2	AGAAAGGATATTGGGCAGCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1034-1038 bp	1.0	83.3
rpl32-trnL-UAG_p3	AGAAAGGATATTGGGCAGCG	CCCCTTTTCCGACGAATCAT	50.0	50.0	58.0	58.0	1328-1334 bp	1.0	82.5
rpl32-trnL-UAG_p4	AGAAAGGATATTGGGCAGCG	AAAAGGCCCGAAGTAGCAAA	50.0	45.0	58.0	58.0	1552-1558 bp	1.0	82.4
rpl32-trnL-UAG_p5	AGAAAGGATATTGGGCAGCG	AAAGGCCCGAAGTAGCAAAA	50.0	45.0	58.0	58.0	1551-1557 bp	1.0	82.4