Hotspot

ndhF-rpl32

SSC · 112397-113490 · 1094 bp

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Pi 0.0232

MarkerSeek score 65.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1094
variable_sites: 36
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCGGTGGGAAAAAGTA	TTTTGAGATAGAAGTACGTTTTT	45.0	26.1	57.9	52.4	1016-1229 bp	1.0	46.5
ndhF-rpl32_p2	TGTTGCGGTGGGAAAAAGTA	TTTTTGAGATAGAAGTACGTTTT	45.0	26.1	57.9	52.4	1017-1230 bp	1.0	46.5
ndhF-rpl32_p3	TGTTGCGGTGGGAAAAAGTA	TTTTTGAGATAGAAGTACGTTTTT	45.0	25.0	57.9	53.2	1017-1230 bp	1.0	46.5
ndhF-rpl32_p4	ATCTTTTGTTGCGGTGGGAA	TTTTGAGATAGAAGTACGTTTTT	45.0	26.1	58.3	52.4	1022-1235 bp	1.0	46.5
ndhF-rpl32_p5	ATCTTTTGTTGCGGTGGGAA	TTTTTGAGATAGAAGTACGTTTT	45.0	26.1	58.3	52.4	1023-1236 bp	1.0	46.5