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rps3-rpl22

LSC · 83815-83942 · 128 bp

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Pi 0.0469

MarkerSeek score 71.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 9
pi: 0.0469
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	ACCAAGTCTGAAGCCAAGTG	GACACAGAAGAAGAATGTTTCGA	50.0	39.1	58.0	57.1	197-198 bp	1.0	52.6
rps3-rpl22_p2	CACCAAGTCTGAAGCCAAGT	GACACAGAAGAAGAATGTTTCGA	50.0	39.1	58.0	57.1	198-199 bp	1.0	52.6
rps3-rpl22_p3	ACCAAGTCTGAAGCCAAGTG	AGACACAGAAGAAGAATGTTTCG	50.0	39.1	58.0	57.1	198-199 bp	1.0	52.5
rps3-rpl22_p4	GAATGATGCCTTTGGGTTGC	GACACAGAAGAAGAATGTTTCGA	50.0	39.1	58.0	57.1	217-218 bp	1.0	52.3
rps3-rpl22_p5	GAATGATGCCTTTGGGTTGC	AGACACAGAAGAAGAATGTTTCG	50.0	39.1	58.0	57.1	218-219 bp	1.0	52.2