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rps4-trnT-UGU

LSC · 46209-46706 · 498 bp

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Pi 0.0295

MarkerSeek score 69.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 22
pi: 0.0295
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GAAACGAGGCCCTCTGTAAC	GCATTTGTAATGCGGTGGTC	55.0	50.0	58.3	58.1	570-587 bp	1.0	80.6
rps4-trnT-UGU_p2	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	588-605 bp	1.0	80.2
rps4-trnT-UGU_p3	TTTTTGAAACGAGGCCCTCT	GCATTTGTAATGCGGTGGTC	45.0	50.0	57.6	58.1	575-592 bp	1.0	80.0
rps4-trnT-UGU_p4	TTTTGAAACGAGGCCCTCTG	GCATTTGTAATGCGGTGGTC	50.0	50.0	58.4	58.1	574-591 bp	1.0	79.7
rps4-trnT-UGU_p5	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	593-610 bp	1.0	79.5