Hotspot

trnY-GUA-trnE-UUC

LSC · 30628-30685 · 58 bp

Back to Procris

Pi 0.0345

MarkerSeek score 66.00

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 3
pi: 0.0345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGATCGGCGAATTATTGGG	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.1	57.9	237 bp	1.0	79.4
trnY-GUA-trnE-UUC_p2	CATGGTGGATCGGCGAATTA	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.1	57.9	242 bp	1.0	79.2
trnY-GUA-trnE-UUC_p3	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	201 bp	1.0	78.5
trnY-GUA-trnE-UUC_p4	ACCAGCGTAGACATATTGCC	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	202 bp	1.0	78.5
trnY-GUA-trnE-UUC_p5	TGGATCGGCGAATTATTGGG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	58.1	58.3	203 bp	1.0	78.2