Hotspot

trnK-UUU-rps16

LSC · 4235-4907 · 673 bp

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Pi 0.0278

MarkerSeek score 67.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 27
pi: 0.0278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	753-758 bp	1.0	82.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.2	58.0	752-757 bp	1.0	82.4
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	ATTTCAAAGAAGGCGGGTGT	50.0	45.0	58.3	58.0	762-767 bp	1.0	81.5
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCAAAGAAGGCGGGTGTTTT	50.0	45.0	58.2	58.2	749-754 bp	1.0	80.6
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTCAAAGAAGGCGGGTGTTT	50.0	45.0	58.2	58.2	750-755 bp	1.0	80.6