Hotspot

rpoC1

LSC · 20966-23758 · 2793 bp

Back to Pritchardia

Pi 0.000358

MarkerSeek score 56.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2793
variable_sites: 1
pi: 0.000358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.3	58.0	2953 bp	1.0	77.4
rpoC1_p2	CTGGAGAGACCCGAATTCCT	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.5	58.0	2948 bp	1.0	75.6
rpoC1_p3	TGAAACTGGAGAGACCCGAA	GCTCGTTCGAGAACTACGAT	50.0	50.0	58.3	57.5	2951 bp	1.0	74.1
rpoC1_p4	ACTGGAGAGACCCGAATTCC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.8	58.0	2949 bp	1.0	73.3
rpoC1_p5	TGGAGAGACCCGAATTCCTA	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.2	58.0	2947 bp	1.0	73.0