Hotspot

ndhI-ndhA

SSC · 121958-122060 · 103 bp

Back to Pritchardia

Pi 0

MarkerSeek score 54.70

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	ACCTTGCAGCTCGTATTGTT	TGACAACTTCTTCCCAACTTGT	45.0	40.9	57.8	58.0	181-196 bp	1.0	66.2
ndhI-ndhA_p2	ACCTTGCAGCTCGTATTGTT	TGCCTAGGATAAGAATGGACCA	45.0	45.5	57.8	58.3	259-274 bp	1.0	63.8
ndhI-ndhA_p3	ACCTTGCAGCTCGTATTGTT	AGATGGACTTTGCCTAGGAT	45.0	45.0	57.8	55.6	269-284 bp	1.0	63.2
ndhI-ndhA_p4	ACCTTGCAGCTCGTATTGTT	GCCTAGGATAAGAATGGACCA	45.0	47.6	57.8	56.6	258-273 bp	1.0	63.1
ndhI-ndhA_p5	ACCTTGCAGCTCGTATTGTT	TGCCTAGGATAAGAATGGACC	45.0	47.6	57.8	56.6	259-274 bp	1.0	63.1