Hotspot

trnD-GUC-trnY-GUA

LSC · 31518-31900 · 383 bp

Back to Pritchardia

Pi 0.0104

MarkerSeek score 60.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 4
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	57.9	58.0	562 bp	1.0	79.7
trnD-GUC-trnY-GUA_p2	GTGCTCTGACCGATTGAACT	AATTCTTCCTGGGTCGATGC	50.0	50.0	57.9	58.0	505 bp	1.0	79.6
trnD-GUC-trnY-GUA_p3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	55.0	55.0	57.9	58.4	529 bp	1.0	77.2
trnD-GUC-trnY-GUA_p4	ATCGGATCCTAGTTCGGGAC	AATTCTTCCTGGGTCGATGC	55.0	50.0	58.4	58.0	565 bp	1.0	77.2
trnD-GUC-trnY-GUA_p5	CATCGGATCCTAGTTCGGGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	58.4	58.0	566 bp	1.0	77.2