Hotspot

ycf1

SSC · 127533-133190 · 5658 bp

Back to Pristimera

Pi 0.0225

MarkerSeek score 53.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5658
variable_sites: 188
pi: 0.0225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCGCCATTTTTGTGGTCCT	TTCACTGACCGAAGCAACAA	45.0	45.0	58.3	58.0	764 bp	0.333333	50.7
ycf1_p2	TTCGCCATTTTTGTGGTCCT	AACGATTCATGAATCCGCGA	45.0	45.0	58.3	58.1	815 bp	0.333333	50.4
ycf1_p3	TTCGCCATTTTTGTGGTCCT	TCTTCACTGACCGAAGCAAC	45.0	50.0	58.3	58.1	766 bp	0.333333	49.8
ycf1_p4	TTCGCCATTTTTGTGGTCCT	ACGATTCATGAATCCGCGAT	45.0	45.0	58.3	57.8	814 bp	0.333333	49.7
ycf1_p5	TCATGGTGGAAGTAATGGGC	TTCACTGACCGAAGCAACAA	50.0	45.0	57.6	58.0	674 bp	0.333333	49.6