Hotspot

rps15-ycf1

SSC · 127083-127532 · 450 bp

Back to Pristimera

Pi 0.0418

MarkerSeek score 69.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 450
variable_sites: 23
pi: 0.0418
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGTTCTGGTCCATTTACGGT	CAGGTCGTCTGGCTGTAAAA	50.0	50.0	57.9	57.8	1576-1629 bp	1.0	84.6
rps15-ycf1_p2	CCAACGTGTTACATAGGGCA	CAGGTCGTCTGGCTGTAAAA	50.0	50.0	57.9	57.8	1523-1576 bp	1.0	84.6
rps15-ycf1_p3	GAGTCGAAGAACACCATGCA	CAGGTCGTCTGGCTGTAAAA	50.0	50.0	58.2	57.8	1397-1450 bp	1.0	83.9
rps15-ycf1_p4	TGAGTCGAAGAACACCATGC	CAGGTCGTCTGGCTGTAAAA	50.0	50.0	58.2	57.8	1398-1451 bp	1.0	83.9
rps15-ycf1_p5	ACAAATAAGCCAGCAGTCGT	CAGGTCGTCTGGCTGTAAAA	45.0	50.0	57.8	57.8	1185 bp	0.333333	50.5