Hotspot

rpl32-trnL-UAG

SSC · 117225-117717 · 493 bp

Back to Pristimera

Pi 0.0407
MarkerSeek score 72.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
493
variable_sites
29
pi
0.0407
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGCTTGACCTTGCTCGAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 972-981 bp 0.666667 69.3
rpl32-trnL-UAG_p2 AGCTTGACCTTGCTCGAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 971-980 bp 0.666667 69.3
rpl32-trnL-UAG_p3 AGCTTGACCTTGCTCGAAAA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 972-981 bp 0.666667 69.3
rpl32-trnL-UAG_p4 AGCGAAATCCCTTGCTACAG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 607 bp 0.333333 53.0
rpl32-trnL-UAG_p5 AGCGAAATCCCTTGCTACAG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 608 bp 0.333333 53.0