Hotspot

petD-rpoA

LSC · 80748-80938 · 191 bp

Back to Pristimera

Pi 0.0456

MarkerSeek score 83.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 13
pi: 0.0456
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	ATTGGTACTGTAGTGGCCCT	TCACATTGAAGACGTGAAACA	50.0	38.1	58.0	56.1	350-363 bp	1.0	62.9
petD-rpoA_p2	GGTTGGGTATTGGAGCAACA	TCACATTGAAGACGTGAAACA	50.0	38.1	58.1	56.1	328-341 bp	1.0	62.9
petD-rpoA_p3	TGGTTGGGTATTGGAGCAAC	TCACATTGAAGACGTGAAACA	50.0	38.1	58.1	56.1	329-342 bp	1.0	62.9
petD-rpoA_p4	GGTACTGTAGTGGCCCTTTG	TCACATTGAAGACGTGAAACA	55.0	38.1	57.9	56.1	347-360 bp	1.0	62.4
petD-rpoA_p5	GTACTGTAGTGGCCCTTTGG	TCACATTGAAGACGTGAAACA	55.0	38.1	57.9	56.1	346-359 bp	1.0	62.4