Hotspot

rps18-rpl20

LSC · 71231-71479 · 249 bp

Back to Pristimera

Pi 0.0321

MarkerSeek score 67.90

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 12
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GACTGCTAGAACTGCTGGTC	AAATACTTGCGCAAATGGCT	55.0	40.0	58.0	57.0	360-380 bp	1.0	76.0
rps18-rpl20_p2	GAGTCGACTGCTAGAACTGC	AAATACTTGCGCAAATGGCT	55.0	40.0	58.1	57.0	365-385 bp	1.0	75.2
rps18-rpl20_p3	CGAGTCGACTGCTAGAACTG	AAATACTTGCGCAAATGGCT	55.0	40.0	57.8	57.0	366-386 bp	1.0	74.6
rps18-rpl20_p4	GACTGCTAGAACTGCTGGTC	AAAATACTTGCGCAAATGGCT	55.0	38.1	58.0	57.7	361-381 bp	1.0	73.8
rps18-rpl20_p5	ACTGCTAGAACTGCTGGTCT	AAATACTTGCGCAAATGGCT	50.0	40.0	58.4	57.0	359-379 bp	1.0	73.2